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Patients in both study arms received folic acid, vitamin B12, and dexamethasone [see Dosage and Administration (2.4)]
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They were then lysed using RIPA lysis buffer containing 0.1% (w/v) SDS, 1% (w/v) Triton X-100, 150 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Tris−HCl (pH 8), and protease inhibitor cocktail, with sonication
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