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Urology 49 , 644–651 (1997)
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Cell Cultures The CaCo-2 cell line, derived from human Caucasian colon adenocarcinoma from the American Type Culture Collection (ATCC, Manassas, VA, USA), was cultured in Dulbecco’s Modified Eagle’s Medium Advance (DMEM-Adv, Thermo Fisher Scientific, Rodano, MI, Italy) supplemented with 10% FBS, 2 mM L-glutamine, and 1% penicillin–streptomycin at 37 °C in an incubator [28]
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