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Although the natural defense design appears to be intended to stop virus attachment and infection, the release of cytokines requires that the system from the cell level to the systemic level have the ability to modulate and moderate the effect that cytokines like tumor necrosis factor (TNF) have on the cells of the entire system

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Western Blot Western blotting was carried out as described previously [21,22], and brain homogenates were lysed on ice for 30 min in 100 mL of lysis buffer (120 mM NaCl, 40 mM Tris (pH 8), and 0.1% NP40) and centrifuged at 12,000 rpm for 30 min