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The following day, sections were washed 6 times for 5 mins each in 0.01 M PBS, and incubated with the appropriate secondary antibodies conjugated to AlexaFluor 488 (donkey anti-rabbit IgG-conjugated Invitrogen, CA, 1:500 in PBST), or AlexaFluor 647 (goat anti-rabbit IgG-conjugated, Invitrogen, CA, 1:500 in PBST) for 2 hours at room temperature in dark
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Durรฉe de la cure:1 mois
2021;41(1) doi: 10.1042/bsr20201920.BSR20201920 [DOI] [PMC free article] [PubMed] [Google Scholar] 15.Wu M., Shang X., Sun Y., Wu J., Liu G
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