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For RPA foci, cells were incubated for 3 h after irradiation and then pre-extracted with buffer I (10 mM PIPES pH 7.0, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl2, 1 mM EGTA and 0.5% Triton X-100), followed by buffer II (10 mM Tris pH 7.5, 10 mM NaCl, 3 mM MgCl2, 1% Tween-40 and 0.5% sodium deoxycholate), as previously 42

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Option:SINGLE

Apply serum to targeted areas (abdomen, thighs, hips, or arms)