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EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell lines All cell lines were maintained in DMEM/F-12 (refer formulation to Thermo Fisher Scientific 11320) based medium supplemented with 10% FBS and 100 units per ml penicillin and 100 μg per ml streptomycin, and were cultured in a 37°C incubator at 20% oxygen
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Despite some limitations, such as efficient delivery and safety, CRISPR–Cas9 is still the most convenient tool for gene-editing purposes 34,35
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