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The protein:ligand complex was crystallized after 3 days of incubation at 20 °C in 20 mM zinc chloride and 20% (w/v) PEG 3,350 by the hanging-drop vapor diffusion method (2 µL protein-ligand complex:2 µL crystallization solution)
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Treatment with etamicastat and blood pressure measurements Acute treatment Etamicastat (10 mg/kg), synthesized in the Department of Chemistry, BIAL - Portela & Cª, S.A
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